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Session 3: Preclinical studies of herbal and traditional medicine
KEYNOTE LECTURE

Phenolic Constituents Analysis and Activity Evaluation in Two Kinds

of Traditional Chinese Medicines

Tao Wang, Yi Zhang, Jian Li, Xuefeng Liu, Lifeng Han, Erwei Liu

Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine
312 Anshanxi Road, Nankai District, Tianjin, 300193, China.
Corresponding author: wangtao@tjutcm.edu.cn

Background and rationale: Mango leaves and sophora flower bud are heat-relieve herbs
and used for diabetes clinical treatment and have been successfully used to prevent and
control diabetes.
Objectives: To reveal the active phenolic compounds and possible anti-diabetes mecha-
nism of Mango leaves. To establish the phenolic compounds quality control method and
confirm the best harvest period of sophora flower bud.
Methods: Type 2 genetic diabetes animal model and cells were used to find active
compounds and clarify the possible mechanism. HPLC-QQQ-MS was used to confirm the best
harvest period of sophora flower bud.
Results and Conclusion: Ethanolic extract of Mango leave dose-dependently decreased
serum glucose and triglyceride in KK-Ay mice. Our in vitro and in vivo investigations revealed
that the effect of Mango leave extract (ME) on glucose and lipid homeostasis is mediated, at
least in part, through PI3K/AKT and AMPK signaling pathway. ME up-regulated the expres-
sion of PI3K, AKT and GYS genes by 2.0 fold, 3.2 fold, and 2.7 fold, respectively, leading to a
decrease in glucose level. On the other hand, ME up-regulated AMPK and altered lipid
metabolism. ME also down-regulated ACC (2.8 fold), HSL (1.6 fold), FAS (1.8 fold) and PPAR-
γ (4.0 fold). From the active fraction, the active compounds of ME can inhibit the TG accumu-
lation in HepG2 cells and the action mechanism maybe related to LKB1-mediated and AMP/ATP
ratio-mediated AMPK activation.
HPLC-QQQ-MS was used to analysis 14 main compounds concluding flavonoids, matol
glycoside, and saponins of S. japonica from different places and different growing period. As
a result, a multi-component quantitative analysis method was established and the best har-
vest period was confirmed.
Key words: Traditional Chinese Medicine, Mango leave, sophora flower bud, anti-diabe-
tes, quality control