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650 วารสารการแพทย์แผนไทยและการแพทย์ ทางเลือก ปีที่ 21 ฉบับที่ 3 กันยายน-ธันวาคม 2566

Antibacterial and Antioxidant Activities of Shallot (Allium ascalonicum)
Extract in Lablae District, Uttaradit Province

†
*,§
Nicharee Jaikhamwang , Thananchai Juapprasop , Natkritta Boonprakob ‡
Public Health Program, Faculty of Science and Technology, Uttaradit Rajabhat University, Ta-It Sub-District,
*
Muang Uttaradit District, Uttaradit 53000, Thailand
Fort Pichai Dab Hak Hospital, Uttaradit Province, Tha Sao Sub-District, Mueang Uttaradit District, Uttaradit 53000, Thailand
†
Program in Chemistry, Faculty of Science and Technology, Uttaradit Rajabhat University, Ta-It Sub-District,
‡
Muang Uttaradit District, Uttaradit 53000, Thailand
§ Corresponding author: jkwnicharee@uru.ac.th
Abstract
Introduction and objective: Shallot (Allium ascalonicum L.) is an economic crop in Uttaradit province.
It is an herb that has important compounds and antioxidant properties as well as anti-inflammatory and antibacte-
rial effects. This experimental research aimed to investigate the antibacterial and antioxidant activities of shallot
extract in Uttaradit’s Lablae district.
Methods: Shallot samples were collected from Lablae district for the study of their botanical characteristics
and quality. Shallot extraction was undertaken with such solvents as water, ethanol and methanol. The crude extracts
were analyzed for the amounts of phenolic compounds and quercetin, and tested for antioxidant and antibacterial
activities.
Results: The percent yields of shallot extracts from water, ethanol and methanol extraction were 9.07%,
8.86% and 11.53%, respectively; and the amounts of phenolic compounds obtained were 33.67, 41.29 and 13.67
mg/mL, respectively. The yields of quercetin were 0.10 ± 0.01, 0.08 ± 0.01 and 0.14 ± 0.01 %w/w of DW, respec-
tively, while the percent inhibitions (DPPH scavenging activity) were 64.09%, 41.53% and 60.89%, respectively.
The analysis of the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC)
showed that the water shallot extract had the best antibacterial activity with MIC and MBC for S. aureus DMST at
10 and 10 mg/mL, S. epidermidis DMST 15505 at 2.5 and 2.5 mg/mL and B. cereus DMST 5040 at 5 and 5 mg/
mL, respectively.
Discussion: The water shallot extract had the best activity against gram-positive bacteria found on the hu-
man skin and the best antioxidant activity that may be attributed to compounds other than phenolic compounds and
flavonoids. Although they are present in small quantities, such additional compounds may contribute to enhancing
the antioxidant properties.
Conclusion and Recommendation: Shallot extract had antioxidant properties and antibacterial activity
against S. aureus, S. epidermidis, and B. cereus. This knowledge can be utilized for further development of herbal
products with an additional value for shallots from Uttaradit province.

Key words: shallot extract, phenolic compounds, quercetin, anti-bacterial activity

บทน�ำและวัตถุประสงค์ aureus ที่ส่งผลท�าให้เกิดการอักเสบของผิวหนัง

โรคผิวหนังเป็นปัญหาที่ส�าคัญทางสาธารณสุข และเป็นสาเหตุของโรคฝีหนองได้ถึง 80% แบคทีเรีย

และส่งผลกระทบต่อทุกเพศทุกวัย โดยเฉพาะอย่าง ประจ�าถิ่น เช่น Staphylococcus epidermidis ซึ่ง
ยิ่งโรคผิวหนังที่เกิดจากการติดเชื้อแบคทีเรีย เช่น อาจเป็นสาเหตุของการเกิดสิว รวมถึงการติดเชื้อบน
แบคทีเรียแกรมบวก ในกลุ่ม Staphylococcus ผิวสัมผัส เช่น Bacillus cereus แม้ปัจจุบันจะมีการ